Effect of urinary kallidinogenase on the expression of bradykinin receptor B1 and B2 after ischemia-reperfusion injury in rats / 中国脑血管病杂志

ABSTRACT

Objective: To observe the effects of different doses of human urinary Kallidinogenase (HUK) on the expression of bradykinin 1 receptor (B1R) and bradykinin 2 receptor (B2R) in SD rats with middle cerebral artery occlusion(MCAO). Methods: The right MCAO model of SD rats was established by modified Zea Longa thread thrombus method. The 25 successfully prepared SD rats were randomly divided into five groups (5 in each group)the Sham group,the I/R model group (I/R +saline group),the HUK low dose treatment group (I/R + LDP group),the HUK medium dose treatment group (I/R + MDP group) and the HUK high dose treatment group (I/R + HDP group). After 30 min,each group was given HUK (the I/R + LDP group3. 50 × 10-3 PNAU/kg;the I/R + MDP group8. 75 × 10-3 PNAU/kg;the I/R + HDP group 17.50 × 10-3 PNAU/kg) or saline tail vein injection for continuous 7 days. The samples were taken after regular injection once a day. The relative expression of mRNA in the marginal area of infarction was detected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR),and microvascular growth density (MVD) was measured by vWF factor inimuno -fluorescence (FITC). Results (1)B1R mRNA expression;Compared with I/R + saline group, the mRNA expression of B1R in I/R + LDP group,I/R + MDP group,I/R + HDP group was all up-regulated ([0. 34 ± 0. 05], [0. 35 ± 0. 04], [0. 47 ± 0. 03] vs. [0.23 ±0.05],all P <0.05);Compared with I/R + LDP group and I/R + MDP group,the mRNA expression of B1R in I/R +HDP group was was all up-regulated (all P<0.05). (2)B2R mRiNA expressionCompared with Sham group, the mRNA expression of B2R in I/R + saline group was up-regulated([0.33 ±0.01]vs. [0.23 ± 0. 02],P <0. 05);Compared with I/R + saline group, the mRNA expression of B2R in I/R + LDP group, I/R +MDP group, I/R + HDP group was all up-regulated ([0. 49 ± 0. 02], [0. 52 ±0. 04], [0. 71 ± 0. 03], respectively,all P < 0. 05); Compared with I/R + LDP group and I/R + MDP group, the mRNA expression of B2R in I/R + HDP group was was all up-regulated (all P <0. 05). (3) Compared with Sham group, the microvessel density(MVD)in I/R + saline group was increased ([169 ±6]vs. [74 ± 12],P < 0.01);Compared with I/R + saline group,the MVD in I/R + LDP group,I/R + MDP group, I/R + HDP group was all increased([240 ±9], [252 ±9], [349 ± 17].respectively,all P<0.01);Compared with I/R + LDP group and I/R + MDP group, the MVD in I/R + HDP group was increased (all P < 0. 01). Conclusion: A certain dose of HUK could upregulate the expression of Bl R and B2R in MCAO rats to promote vascular regeneration,and the effect of high dose HUK on neovascularization was more obvious.