Expression differences of poly adenosine diphosphate ribose polymerase 1 in the human intracranial ruptured and unruptured aneurysm walls / 中国脑血管病杂志

ABSTRACT

Objective To investigate the expression differences of poly adenosine diphosphate ribose polymerase 1 (PARP-1) in the human intracranial ruptured and unruptured aneurysm walls. Methods Consecutive patients with intracranial aneurysm treated with craniotomy clipping surgery in Drum Tower Hospital, Nanjing University School of Medicine (n = 1) and General Hospital of Eastern War Zone (n = 12) from January 2014 to September 2018 were enrolled retrospectively. Seven of them were ruptured aneurysms and 6 were unruptured aneurysms. The expression of PARP-1 in aneurysm walls was detected by immunohistochemistry. Results Immunohistochemistry staining showed that the positive expression of PARP-1 was brown-yellow and mainly located in nuclei; in the unruptured aneurysm walls,the number of PARP-1 positive cells was small, the distribution was sparse,and it expressed in the whole layer of the aneurysm wall;in the ruptured aneurysm walls,PARP-1 positive cells were densely distributed in the outer membrane of the vessel walls. Statistical analysis showed that the positive expression of PARP-1 in the ruptured aneurysm walls was higher than that in the unruptured aneurysm walls. The difference was statistically significant(160 ± 19 vs. 56 ± 14,1 = 4. 291, P < 0. 01) . Conclusion The expression of PARP-1 in the ruptured aneurysm walls is significantly higher than that in the unruptured aneurysms,which may play a role in the rupture of intracranial aneurysms.