Effect of mannitol combined with bone marrow mesenchymal stem cells on cognitive function and the expression of brain-derived neurotrophic factor of vascular dementia rats / 中国脑血管病杂志

ABSTRACT

Objective: To investigate the effect of mannitol combined with the vein graft supported with bone marrow mesenchymal stem cell (BMSC) on the cognitive function and the expression of brain-derived neurotrophic factor (BDNF) in brain tissue of vascular dementia (VD) rats. Methods: The BMSCs of rat were isolated and purified by whole bone marrow culture and adherence screening methods. Seventy nine healthy male SD rats were randomly assigned to sham operation group (n = 10) and model (n = 69) groups. A VD model was established by the ligation of the bilateral common carotid arteries at an interval of 3 days. Four weeks after modeling, the 32 rats in the model group were randomly allocated into mannitol pretreatment BMSC transplantation group (tail intravenous injection of mannitol 1.5 g/kg, tail intravenous injection of 1 × 106 mL BMSCs 1 mL after pretreatment for 1 to 30 minutes), BMSC transplantation group (injecting the same amount of BMSCs but not mannitol), and medium control group (injecting the same amount of basal medium). After removing the dead rats, there were 9, 11, and 8 rats, respectively in the above 3 groups. Seven rats were included in the sham operation group. Morris water maze test was used to detect cognitive function of the rats, and an enzyme-linked immunosorbent assay was used to detect the BDNF content in brain tissue 4 weeks after intervention. Results: Circled Digit oneComparing the 4 groups at the same time points, the escape latency in the BMSC transplantation group was shorter than that in the medium control group (all P 0.05). Circled Digit twoThe BDNF content in the BMSC transplantation group was significantly higher than that in the medium control group in the frontal cortical and hippocampal regions (P < 0.01). The mannitol pretreatment BMSC transplantation group was more significantly higher than the BMSC group (P < 0.01), however, they were lower than the level in the sham operation group (P < 0.01). Conclusion: Compared with intravenous injection of BMSCs alone, the improvement of cognitive function of the VD rat model treated with mannitol combined with BMSC transplantation is much better; the BDNF expression in cortex and hippocampus is higher.