Role of endothelial progenitor cells in healing aneurysms with flow diverting stents / 中国脑血管病杂志

ABSTRACT

Objective: To investigate whether endothelial progenitor cells (EPCs) involved in the early healing process of aneurysms with flow diverting stents and the mechanisms. Methods: Five clean rabbits were chosen to prepare the animal model of aneurysm on the right carotid artery. After EPCs were cultured autologously, they were double labeled by Hoechst 33342 and carboxyfluorescein diacetate succinimidylester (CFSE). The aneurysms of rabbits were treated using flow diverting stents. The labeled EPCs were retransfused autologously via veins. EPCs involving in the intimai repair of aneurysms were observed with Cell tracking technology after 2 weeks. In combination of scanning electron microscopy, pathology and immunohistochemistry, EPCs involving in the process of intimal repair were analyzed. Results: Circled digit oneTwo weeks after simple flow diverting stents treatment, the rabbits were reexamined. Their aneurysms did not visualized completely (5/5). The aneurysms were obliferated completely, and the aneurysm necks were covered with neointima. Circled digit twoHoechst33342 and CFSE double-labeled positive cells were found in aneurysm sections. In the aneurysm sections of 2 experimental animals (2/5) , the positive cells were in the neointima. Circled digit threeScanning electron microscopy and immunohistochemistry results suggested that only a few amount of endothelial cells repaired the intimai surface. Conclusion: EPCs involved in the process of early intimal healing of aneurysms.