Preparation and in vitro evaluation of tissue engineered osteochondral integration of multi-layered scaffold / 中国修复重建外科杂志
Chinese Journal of Reparative and Reconstructive Surgery
;
(12): 434-440, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-856800
ABSTRACT
Methods:
According to blend of different components and proportion of acellular cartilage extracellular matrix of pig, nano-hydroxyapatite, and alginate, the osteochondral integration of multi-layered scaffold was prepared by using freeze-drying and physical and chemical cross-linking technology. The cartilage layer was consisted of acellular cartilage extracellular matrix; the middle layer was consisted of acellular cartilage extracellular matrix and alginate; and the bone layer was consisted of nano-hydroxyapatite, alginate, and acellular cartilage extracellular matrix. The biological and mechanics characteristic of the osteochondral integration of multi-layered scaffold were evaluated by morphology observation, scanning electron microscope observation, Micro-CT observation, porosity and pore size determination, water absorption capacity determination, mechanical testing (compression modulus and layer adhesive strength), biocompatibility testing [L929 cell proliferation on scaffold assessed by MTT assay, and growth of green fluorescent protein (GFP)-labeled Sprague Dawley rats' bone marrow mesenchumal stem cells (BMSCs) on scaffolds].
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Reparative and Reconstructive Surgery
Year:
2018
Type:
Article
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