Preliminary study of basis of hepatotoxic substance of ripe fruit of Terminalia chebula Retz. var. tomentella Kurtwith high content screening assay / 中国药理学通报

ABSTRACT

Aim To study the material basis of hepatotoxicity induced by the ripe fruit of Terminalia chebula Retz. var. tomentella Kurt using high content screening. Methods Shikimic acid, benzoic acid, gallic acid, and 1,2,3,4,6-o-pentagalactosyl glucose were applied to HepG2 cells, respectively, and the cells were stained with fluorescent stains such as Hoechst 33342. The imagewas scannedand the collected datawere input into the Assay Template. Finally, the dose-response curves of cell numbers, DNA content, GSH reduction level, ROS content, MMP and other indicators were obtained for different monomers at different concentrations, thereby the hepatotoxicity of the monomers was determined. Results Aspirin and shikimic acid showed negative results. Ticlopidine, benzoic acid, 1,2,3,4,6-o-pentagalloglucose, gallic acid caused a significant decrease in cell number and increase in ROS content. There was a risk of liver-toxicity. Conclusions Gallic acid, benzoic acid, 1,2,3,4,6-o-pentagalactosylglucose have the risk of hepatotoxicity, and the risk of hepatotoxicity caused by gallic acid is the largest. Basically, gallic acid is safer when administered at concentrations below 50 mg·L-1