Effect of Bmi-1 on invasion and metastasis of renal cancer cells and its related mechanism / 中国药理学通报

ABSTRACT

Aim To detect the effect of B-cell-specific moloney murine leukemia virus in section site 1 (Bmi-1) on the migration and invasion of renal cancer cell line 786-0 and ACHN and its possible mechanism. Methods siRNA interfered with the expression of Bmi-1 in renal cancer cells, and the effects of Bmi-1 on the migration and invasion of renal cancer cells were detected by scratch healing and Transwell chamber assay. The expression of invasion and metastasis related proteins in 786-0 and ACHN after Bmi-1 knockdown were detected by Western blot. Results Protein expression of Bmi-1 increased in renal cancer cells. In vitro synthesis of specific Bmi-1 gene siRNA significantly inhibited the expression of Bmi-1 in 786-0 and ACHN cells. After silencing Bmi-1, the scratch healing ability and migration and invasion activity of renal cancer cells were significantly lower than those of control cells. The results of Western blot showed that epithelial markers E-cadherin increased after Bmi-1 silencing, while the expression of Vimentin decreased, and the phosphorylation level of Akt was also reduced. Conclusions Silencing Bmi-1 inhibits the migration and invasion of renal cancer cells, and its effect is related to the activation of Akt signaling pathway.