Effects of integrin av ß3 inhibitor CycIo-RGDfK on epithelial- mesenchymal transition of breast epithelial cells induced by fibronectin / 中国药理学通报

ABSTRACT

Aim To investigate the effect of integrin av ß3 inhibitor (Cyclo-RGDfK) on fibronectin (F N) - induced epithelial-mesenchymal transition in human mammary epithelial cells MCF-10A. Methods M C F - 10A cells were cultured in vitro, and the model of epithelial-mesenchymal transition (E M T) induced by FN was established. The expressions of otv integrin, ß3 integrin, epithelial marker E-cadherin and mesenchymal markers N-cadherin and Vimentin were measured by Western blot. The migration ability and invasion ability of cells were detected by Scratch repair experiment and Transwell chamber experiment. Results Western blot results showed that the expressions of ay integrin, ß3 integrin, N-cadherin, and Vimentin significantly were up-regulated and the expression of E-cadherin protein down-regulated after FN treatment for 48 h. After administration of Cyclo-RGDfK, the increased expression of N-cadherin and Vimentin and decreased expression of E-cadherin induced by FN were significantly reversed. Similarly, the results of Scratch repair experiments and Transwell experiments showed that FN significantly enhanced the ability of MCF-10A cells to migrate and invade, and Cyclo-RGDfK significantly inhibited the induction of FN. Conclusions Cyclo-RGDfK can inhibit the process of EMT induced by FN and reduce the invasion and migration of MCF-10A, which may be a potential drug for the treatment of breast cancer metastasis.