Sodium aescinate alleviates CC14-induced liver fibrosis in rats by inhibiting phosphorylation of 4EBP1 / 中国药理学通报

ABSTRACT

Aim To study the inhibitory effects of sodium aescinate in liver fibrosis induced by carbon tetrachloride (CCl4 ). Methods Forty-one male SD rats were recruited in this study and randomized into control group (n = 5), model group (n = 18), and sodium aescinate treated group (n = 18) . Masson staining was performed for collagen fiber detecting, and IHC staining was conducted for accessing the expression of interest proteins in rat liver. MTT and apoptosis assay were performed to evaluate the effects of sodium aescinate on HSC-T6 cells. The expression of interest proteins was detected by immunoblotting. Results Sodium aescinate alleviated the liver fibrosis induced by CCl4 through proliferation inhibition and apoptosis induction in HSC-T6 cells. Sodium aescinate also down-regulated the phosphorylation of 4EBP1, the expression of collagen I and collagen III. Conclusion Sodium aescinate alleviates liver fibrosis induced by CCl4,.