Isorhamnetin prevents pc12 cell from rotenone-induced iiyury via pdk/akt/gsk-3p/creb signaling pathway / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12): 272-276, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-857029
ABSTRACT
Aim To investigate whether isorhamnetin could protect against rotenone-induced PC 12 cells injury via regulating PI3K/Akt/GSK-3p/CREB pathway. Methods PC 12 cell viability was determined by MTT and LDH assays. The expressions of Akt,p-Akt,GSK-3p,p-GSK-3p,CREB and p-CREB were measured by Western blot. Results The cell viability and level of phospho-CREB significantly decreased in PC 12 cells exposed to rotenone when compared to control group. Both the cell viability and the expression of phospho-CREB in cells pretreated with isorhamnetin were higher than those of cells exposed to rotenone alone. Moreover, pretreatment of PC 12 cells with isorhamnetin enhanced phosphorylation of Akt and GSK-3fi. The addition of LY294002 could suppress the phosphorylation of Akt, GSK-3p and CREB, resulting in abolishment of neuroprotective effects of isorhamnetin on cells exposed to rotenone. Conclusion Isorhamnetin might alleviate rotenone-induced PC 12 cells injury partially via the PI3K/Akt/GSK-3 (3/CREB signaling pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmacological Bulletin
Year:
2020
Type:
Article
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