Application of microvascular measurement technology in endothelial removal / 中国药理学通报

ABSTRACT

Aim To improve the reliability of research data of microvascular measurement technology by establishing a research method for the removal of vascular endothelium from isolated arterioles. Methods For this study, 12-wk-old male spontaneously hypertensive rats (SHR) and Wistar-Kyoto ( WKY) rats were selected. Acute separation of the mesenteric artery was performed after measurement of blood pressure. The endothelium was removed by a combination of mechanical (the vascular ring rotated around the electrode tip and gas was injected) and drug (L-NAME and indom-ethacin). Thenthe effects of different concentrations of phenylephrine ( PE), ACh, and sodium nitroprusside (SNP) on the changes of the mesenteric artery diameter in the rats with endothelial integrity and endothelial removal were measured using pressure myocardio-graphy. Results (1) The blood pressure ofSHR rats was significantly higher than that of WKY rats (P could relax the mesenteric artery of SHR and WKY rats in a concentration-dependent manner. When the endothelium was intact, the relaxation of SHR was significantly weaker than that of WKY rats (P < 0. 01 )j Compared with WKY rats, SHR showed a significantly enhanced relaxation when the endothelium was removed (P <0. 01). Conclusions (1) We successfully established a research method for vascular endothelium removal by in vitro arteriolar pressure arthrography. ( 2 ) Hypertensive rats have a significant vasomotor dysfunction.