Role of M A P K / E R K signaling pathway in apoptosis of nasopharyngeal carcinoma cells by Yiqi Jiedu formula / 中国药理学通报

ABSTRACT

;Aim To study the effect of Yiqi Jiedu formula extract on the apoptosis of nasopharyngeal carcinoma cells, and explore the mechanism of its induction of apoptosis from MAPK/ERK signaling pathway. Methods The effect of Yiqi Jiedu Formula extract on the proliferation of CNE1 and CNE2 cells was detected by CCK-8. The apoptosis of CNE1 and CNE2 cells was detected by Hoechst 33342 staining, JC-10 staining and fluorescence double dye flow cytometer staining. The protein expression of CNE1 and CNE2 cells was detected by Western blot. Results Yiqi Jiedu formula extract inhibited the proliferation (P < 0. 05) and induced apoptosis (P <0. 05) of CNE1 and CNE2 cells. After 48 h of drug treatment, the expression of Survivin, XIAP and Bcl-2 decreased, Bax increased, and p-c-Raf, p-MEK and p-ERKl/2 of MAPK/ERK signaling pathway significantly decreased (P < 0. 05). On this basis, after adding activator of MAPK/ERK signaling pathway ISO, the expression of p-c-Raf, p-MEK and p-ERKl/2,Survival, XIAP and Bcl-2 increased, while the expression of Bax decreased compared with the extract of YQ group. Drug-induced apoptosis effects were also reduced. Conclusions Yiqi Jiedu formula extract can induce the apoptosis of nasopharyngeal carcinoma cells, which inhibits the expression of key proteins p-c-Raf, p-MEK and p-ERKl/2 in MAPK/ERK signaling pathway, and then down-regulates the expression of Survivin, XIAP, Bcl-2 and up-regulates the expression of Bax.