Epigallocatechin promotes nuclear translocation of Nrf2 to reduce damage of Aβ25-35 in SH-SY5Y cells / 中国药理学通报

ABSTRACT

Aim To investigate the protective effect of epigallocatechin(EGC) on SH-SY5Y cells induced by Aβ25-35 injury. Methods MTT and LDH assay were employed to investigate the effects of EGC on viability of SH-SY5Y cells. DCFH-DA fluorescent probe was used to detect the intracellular ROS levels, and the activity of SOD, GSH-Px and MDA in cells was measured to investigate the level of oxidative stress in cells. Western blot analysis was used to determine Nrf2, NQ01, HO-1, Prdx6 and Trxl levels in cells. Results SH-SY5Y cells were injured by Aβ25-35 treatment, and the cell viability was significantly reduced. 5, 10 and 20 μmol . L 1 of EGC alleviated Aβ25-35 induced SH-SY5Y cell injury and reduced LDH release. In addition, EGC could reduce the intracellular ROS level induced by Aβ25-35 and inhibit oxidative stress. At the same time, EGC could significantly enhance the expression of Nrf2, NQ01, HO-1, Prdx6 and Trxl after Aβ25-35 injury. Conclusions EGC could inhibit Aβ25-35 -induced SH-SY5Y cell injury, increase the cells antioxidant levels and reduce the neurotoxicity of Aβ25-35 by promoting nuclear translocation of Nrf2.