Study on preparation of curcumin-PLGA nanoparticles and their effect on apoptosis in glioma cells / 中国药理学通报

ABSTRACT

Aim To prepare curcumin-PLGA nanoparticles [curcumin poly(lactic-co-glycolic acid) nanoparticles, Cur-PLGA-NPs ] and evaluate their effects on glioma cells in vitro. Methods C6 cells and U251 cells were subcultured and randomly divided into control group, curcumin group and curcumin-PLGA group. The morphology of Cur-PLGA-NPs was observed by inverted fluorescence microscope and transmission electron microscopy. The particle size of Cur-PLGA-NPs was detected by Malvem particle size potentiometer. The encapsulation efficiency and drug loading rate of Cur-PLGA-NPs were detected by a multi-function microplate reader. The uptake of Cur-PLGA-NPs by C6 cells and U251 cells was observed by fluorescence microscopy. The effect of Cur-PLGA-NPs on the viability of C6 cells and U251 cells was detected by CCK-8. The effect of Cur-PLGA-NPs on apoptosis was assessed by flow cytometry. Apoptosis was detected by Hoechst staining. Results The Cur-PLGA-NPs had an average particle size of (284. 6 ± 9. 0) nm and were spherical. The encapsulation efficiency was (70.712 ±2.615)%, and the drug loading rate was (2.828 ±0.105) %. Compared with control group and curcumin group, C6 cells and U251 cells significantly increased the uptake of Cur-PLGA-NPs (P < 0. 05). Compared with control group and curcumin group, the cell viability of C6 cells and U251 cells in curcumin-PLGA group significantly decreased (P < 0. 05). Compared with control group and curcumin group, the Cur-PLGA-NPs induced apoptosis significantly in C6 cells and U251 cells(P <0. 05). Conclusion Compared with curcumin, Cur-PLGA-NPs can enhance the uptake of drugs by tumor cells, promoting tumor cell apoptosis.