Effect of protein kinase D1 on myocardial inflammation and apoptosis in rats with myocardial infarction / 中国药理学通报

ABSTRACT

Aim To investigate the effect of protein kinase Dl (PKD1) on myocardial inflammation and apoptosis after myocardial infarction and to analyze its molecular mechanism. Methods Forty-five male Wistar rats were randomly divided into three groups sham-operated group, model group and PKD1 group. Rat models of myocardial infarction were reproduced by classical left coronary artery ligation in model group and PKD1 group, while rats in sham group were operated without ligation of coronary artery. The parameters of hemodynamics in rats were assessed, the morphological changes of myocardial tissue were analyzed by HE staining, the apoptotic changes of myocardial cells were analyzed by TUNEL staining, immunohistochemical staining and Western blot, while the changes of myocardial inflammation were analyzed by immunoblotting and real-time quantitative PCR. Results Compared with model group, PKD1 could improve the hemodynamic indexes in rats with myocardial infarction, reduce the injury caused by myocardial infarction, inhibit apoptosis in myocardial tissue, up-regulate the protein expression of Bcl-2, down-regulate the protein expression of caspase-3, Bax, TLR4, TN-C, NF-ΚB p50, NF-ΚB p65 and decrease the mRNA expression of IL-1, NF-ΚB p50 and NF-ΚB p65, and all the differences were statistically significant (P <0. 01). Conclusion PKD1 might have the biological function of inhibiory effect on inflammation and apoptosis induced by myocardial infarction injury.