Banqiao Codonopisis Pilosula improves cognitive dysfunction in AD model rats by PP2A signaling pathway / 中国药理学通报

ABSTRACT

Aim To investigate the protective effect of Banqiao Codonopsis pilosula (BCP) on cognitive function in rats with Alzheimer's disease(AD) induced by Okadaic acid (OA) and its possible mechanism. Methods SD rats were randomly divided into DMSO group, OA group and BCP low, medium, high treatment group. The rats were gavage administered in groups of one week and two weeks. The water maze training was continued for five days before modeling, and modeling was started 24 hours after the training. The bilateral hippocampus of DMSO group was injected with 10% DMSO 1.5 |xL. OA group and BCP treatment group were injected with OA (0. 392 mmol • L"1) 1.5 (iL. The water maze test was used to observe the spatial learning ability of rats. Western blot was used to observe the activity of PP2A, the phosphoryla-tion of Tau protein and the expression of synaptic protein in hippocampus, and the Nissl's staining to observe the changes of Nissl bodies in hippocampus CA1 and CA3. Results Water maze experiments showed that BCP could improve spatial memory impairment in AD rats. Western blot results showed that BCP in-creased PP2A activity, increased synaptic protein expression, and decreased Tau protein phosphorylation. Nissl's staining suggested an increase in the number of Nissl bodies in BCP treatment group. Conclusions BCP can up-regulate PP2A activity, decrease the phosphorylation level of Tau protein, increase the expression of synaptic proteins, and repair damaged neurons.