Effects of ionizing irradiation on ribosome signaling pathway in spleen tissues of mice / 中国药理学通报

ABSTRACT

Aim To clarify the damage mechanisms of ionizing radiation on mouse spleen tissues and analyse differential protein expression in spleen tissues of mice exposed to ionizing radiation injury and normal mice from the perspective of comparative proteomics, in order to analyse the signaling pathways involved in differential proteins. Methods The mouse ionizing radiation injury model was established by 5 Gy 60Co-y ray, and the mouse spleen tissue was extracted in the ionizing radiation group and normal group, then ITRAQ combined with LC-MS/MS detection technology were used to screen differentially expressed proteins of spleen tissues from ionizing radiation group and normal group. Results A total of 17 biological signaling pathways were identified by KEGG pathway enrichment analysis(P <0. 0 5) , in which 37 differential expressed proteins were enriched in the ribosome signaling pathway pathway , including 36 differential expressed proteins downregulated and one differential expressed protein up-regulated. These pathway involved several multiple subunit proteins, such as ribosome proteins, 60S ribosome proteins and 40S ribosome proteins, which were mainly involved in biological processes, such as translation of proteins, structural composition of ribosome and so on. Conclusions Ionizing radiation causes 37 differential proteins involved in ribosome signaling pathway of mouse spleen tissues, including 36 differential expressed proteins down-regulated and one differential expressed protein up-regulated. The disturbance of protein synthesis in spleen tissues is an important mechanism of ionizing radiation injury.