Synergic effect of Chuanxiong Rhizoma and borneol on protecting hippocampus and hypothalamus of rats from global cerebral ischemia reperfusion injury / 中国药理学通报

ABSTRACT

To research the synergic effect of Chuanxiong Rhizoma(CR) and borneol(BO) on pro-tecting hippocampus and hypothalamus of the rats from global cerebral ischemia reperfusion (GCIR) injury. Methods GCIR rats were divided into five groups, i. e. sham, model, CR(1.0 g kg"1), B0(0. 16 g kg"1) and CR + BO groups. The contents of Glu, Gly and GABA in brain microdialysis solution were detected by GC-MS. [Ca2+]i of the two regions were measured using a confocal microscope. The apoptotic rate and ultrastructure were obtained by TUNEL stain and transmission electron microscopy, respectively. Results The monotherapy of CR or BO attenuated Ca2 + over-load and improved ultrastructure in both areas. Their synergy showed a better effect. CR alone reduced the apoptotic rate, increased the levels of GABA in both brain areas, while BO only decreased that of Glu. The combination even enhanced the content of Gly in hypothalamus. Conclusions Obvious synergic effects between CR and BO exist in attenuating neuroceliular ex-citotoxicity and calcium overload, while the anti-apop-totic effect might be excluded.