Ribonucleic acid II induces apoptosis of human leukemia kgla cells through pi3k/akt activation mediated by reactive oxygen / 中国药理学通报

ABSTRACT

Aim To investigate the correlation between ribonucleic acid ? on human leukemia cell lines KG1a and PI3K/Akt signaling pathway by reactive oxygen.Methods The cell cycle and the ROS level of ribonucleic acid ? in human leukemia cell lines were determined by flow cytometry.The levels of p-PI3K, p-Akt, p-MDM2, p21, cyclin D1 and CDK4 were detected by Western blot.The expression levels of protein p-PI3K, p-Akt and p53 after treated with ribonucleic acid ? and NAC were also detected by Western blot.Results The results of FCM revealed that the cell cycle was arrested at G0/G1 phase and ROS pre-vented the arrest of cell cycle.The results also showed that ROS level increased with the concentration of ribonucleic acid ? in KG1a cells.Western blot results showed after treated with ribonucleic acid ?, the expression levels of p-PI3K, p-Akt, p-MDM2, cyclin D1 and CDK4 decreased, while p21 increased in KG1a cells.After treated with ribonucleic acid ? and NAC, the expression levels of p-PI3K and p-Akt increased, while p53 decreased.Conclusion Ribonucleic acid ? can induce the apoptosis of leukemia KG1a cells through ROS-mediated PI3K/Akt signaling pathway.