Study on sodium aescinate inhibiting hepatocellular carcinoma by eif4a1 / 中国药理学通报

ABSTRACT

Aim To investigate the correlation between the expression of EIF4A1 and hepatocellular carcinoma (HCC) and the potential mechanism of sodium aescinate inhibiting the proliferation of hepatoma cell lines. Methods Immunohistochemistry was used to detect the expression level of EIF4A1 in tumor specimens of 80 patients with HCC. The results combined with clinical indicators and follow-up information were used to analyze their relevance. Annexin V-FITC/PI double staining method was employed to detect the effects of sodium aescinate on apoptosis of HepG2 and human L02 cell lines. Transwell migration assay was used to detect the effect of sodium aescinate on the migration of two cell lines. Western blot, qPCR and immunofluorescence were used to detect the expression changes of E1F4A1 of two cell lines after sodium aescinate treat ment. Results The expression of EIF4A1 significantly increased in HCC tissues, and the expression of EIF4A1 was correlated with tumor differentiation, tumor diameter and survival time. Sodium aescinate (40 jxmol • L"1) could significantly promote the apoptosis of liver cancer cell line and inhibit its migration a-bility, but had no effect on normal liver cell line. Sodium aescinate inhibited the growth and proliferation of hepatoma cell line while down-regulated the expression of hepatoma cell line EIF4A1. Conclusions EIF4A1 is associated with the development of HCC, and sodium aescinate can inhibit hepatoma cell line via affecting the expression of EIF4A1.