Expression and role of acid-sensitive ion channel-la in lung tissues of rats with acute lung injury / 中国药理学通报

ABSTRACT

Aim To explore the expression and possible effect of acid sensing ion channel-la( ASICla) in lung tissues of rats with acute lung injury ( ALI). Methods Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups Control group, model group, amiloride group and Dex group. Blood gas analysis of arterial blood, observation of lung histo-pathology were performed and wet/dry ratio was calculated after weighing of lung tissues, then the expression of TNF-a in serum was detected by ELISA, and the expression of ASICla in lung tissues was also detected by qPCR and Western blot. Results Compared with control group, the lung tissue damage was obvious in model group, the wet/dry ratio of lung tissues obviously increased, and the expression level of TNF-a markedly increased (P < 0. 01 ). Lung tissue injury was significantly alleviated in amiloride group and Dex group, the wet/dry ratio of lung tissues obviously decreased , and the expression level of TNF-a significantly decreased (P < 0.01). Immunohistochemistry, qPCR and Western blot results showed that, compared with control group, the expression of ASICla significantly increased in model group (P <0.01), and markedly decreased in amiloride group (P <0.01). Conclusions The expression of ASIC 1 a increases in acute lung injury induced by lipopolysaccharide, which may be involved in the occurrence and development of ALI.