Protective effect of likun preparation no. 1 on bone marrow suppression in mice and its molecular mechanism / 中国药理学通报

ABSTRACT

To explore the protective effectof Likun preparation No.1 on bone marrow suppression injury in mice and the possible mechanism. Methods Sixty male BALB/c mice were randomly divided into normal control group, 5-Fu model group, positive control group(rhG-CSF) and Likun preparation No.1 groups with low, medium and high doses. Myocardial inhibition was established by intraperitoneal injection of 5-fluorouracil once. After seven days of administration, blood routine analysis was performed. Serum levels of TNF-α and IL-2 were detected by ELISA. The expression of p-JAK2 and p-STAT5 protein in spleen tissues were determined by Western blot. Results After given the Likun preparation No.1, the symptoms of diarrhea in mice were alleviated, body weight increased(P < 0.05), and white blood cells, platelets and lymphocytes also increased(P < 0.05). ELISA test showed that the serum level of IL-2 in Likun preparation high-dose group was higher than that in model group(P < 0.05), and the TNF-α content was lower than that in model group(P < 0.05). Western blot analysis showed that compared with model group, the expression of p-JAK2 and p-STAT5 protein in spleen of mice in Likun preparation group was up-regulated(P < 0.05). Conclusions The Likun preparation No.1 has protective effect on 5-Fu-induced myelosuppression in mice. The molecular mechanism may be related to the up-regulation of p-JAK2 and p-STAT5 protein expression, the promotion of IL-2 production and the reduction of TNF-α secretion.