Study of effects of self-made Tongluoquzhuo prescription on non-alcoholic simple fatty liver rats established by two different methods and its mechanisms / 中国药理学通报

ABSTRACT

Aim: To investigate the effect of Tongluoquzhuo prescription on the intervention of non-alcoholic simple fatty liver (NAFL) rats established by chemical injury or a high-fat diet. Methods: Three testing groups, vehicle group, NAFL group and intervention group were set in the experiment of NAFL rats established by carbon tetrachloride (CC14); and those of control group, NAFL group and intervention group were set in the experiment of NAFL rats established by a high-fat diet. Blood biochemical determination, contents of hepatic lipids, CYP enzyme activity, his-topathological observation, and metabolomics analysis were measured. Results: Tongluoquzhuo prescription reduced the hepatic lipids of NAFL rats established by different methods significantly, lowered liver weight and liver index, and had dual-direction regulation of blood triglyceride (TG). CYP enzyme activity was reduced significantly in NAFL rats established by CC14, and raised significantly in intervention group. It may be effected by the pathway of phospholipid to reduce lipid accumulation in the liver of NAFL rats established by CC14, while free fatty acid was the most important factor of the interventional effect in NAFL rats established by a high-fat diet. Conclusions: Tongluoquzhuo prescription has evident effect of protecting liver and reducing lipids in NAFL rats established by different methods. On the other aspect, It shows difference between the main metabolites and metabolic padi-ways involved in the intervention of Tongluo turbidity in NAFL established by different methods. It is suggested that different metabolites are required as biomarkers, although different causes can produce similar hepatic steatosis.