Metabolomics study on effects of compatibility of alcohol extracts of Magnolia officinalis and Polygala tenuifolia on urine metabolites in rats / 中国药理学通报

ABSTRACT

Aim: To study the effect of compatibility of Magnolia officinalis and Polygala tenuifolia on urine metabolites in rats, to explore its characteristic biomar-kers and analyze the related metabolic pathways, and to provide references for exploring the possible mechanisms of Magnolia officinalis relieving gastrointestinal motility disorders induced by Polygala tenuifolia. Methods: Forty male Sprague-Dawley rats were randomly divided into control group, Polygala tenuifolia 1.75 g · kg-1 group, Magnolia officinalis 3.50 g · kg-1 group, and the compatibility group (3.50 + 1.75) g · kg-1, continuously orally administered for three days. Urine was collected within 24 hours after the last administration and detected by UPLC-Q-TOF-MS in each group. The characteristic biomarkers and corresponding metabolic pathways were analyzed or screened by Progenesis QI V2.0, SIMCA-P 14.0, SPSS 20. 0, MetaboAnalyst 3. 0 and other softwares. Results: The metabolites in control group and drug-administered group could be clearly distinguished, and a total of 16 characteristic metabolites, 2-Aminobenzoic acid, D-Glucurono-6, 3-lactone and Homovanillin, were screened out. The main related pathways included Tyrosine metabolism, tryptophan metabolism, vitamin B6 metabolism and primary bile acid biosynthesis. Conclusions: The mechanism of Magnolia officinalis relieving gastrointestinal motility disorders induced by Polygala tenuifolia might be related with Tyrosine metabolism, tryptophan metabolism, primary bile acid biosynthesis and vitamin B6 metabolism.