Polygalasaponin F inhibits secretion of IL-1β and its molecular mechanisms / 中国药理学通报

ABSTRACT

Aim To investigate the effects of polyga-lasaponin F( PS-F) on LPS-induced inflammatory cytokine IL-1β expression in BV2 cells and explore the potential anti-inflammatory mechanism of PS-F. Methods Five groups were included in the experiment, including the control group, the model group and PS-F treatment groups(0. 10, 1.00, 10.00 μmol • L-1). ELISA, quantitative real-time PCR and Western blot were used to assay the mRNA and protein expression of IL-1β. The levels of NLRP3, caspase-1, ASC, caspase-11 protein were examined by Western blot. Results Compared with model group, PS-F treatment groups effectively reduced IL-1β expression in LPS-in-duced inflammatory model in a dose-dependent manner ( P <0. 01 or P <0. 05). Moreover, PS-F significantly inhibited ASC and caspase-11 expression (P < 0. 05 ). Conclusions PS-F inhibited the expression of inflammatory mediator IL-1β by suppressing the activation of NLRP3 inflammasome and inhibiting caspase-11 in LPS-stimulated BV2 cells.