Effect of main active components combination of Astragalus and Angelica on hematopoietic function in mice with bone marrow suppression / 中国药理学通报

ABSTRACT

Aim To explore the effect of the main active components combination of Astragalus promoting hematopoiesis on mouse model of bone marrow hematopoietic suppression. Methods The contents of five main active components such as ferulic acid, astragalo-side IV, formononetin, calycosin and calycosin glycoside were determined after the extract of Astragalus combined with Angelica at 1 1 ratio was prepared by water extraction, thus ascertaining the combination dosage. Mice were randomly divided into blank control, model, active component alone, five active components' combination and Astragalus-Angelica combination groups. The blank control group was given solvent for 7 days; the model group was given the intragastric administration as above and received intraperitoneal injection of cyclophosphamide on day 3 for 3 days; each drug group was treated intragastrically and established the model as above. The peripheral blood, the content of serum hematopoietic growth factor and the colony forming of hematopoietic progenitor cells were measured. Results The active components' combination and Astragalus-Angelica combination could significantly increase the numbers of peripheral blood leukocytes, e-rythrocytes, platelets, the content of hemoglobin and the area of bone marrow hematopoietic tissue (P < 0. 05 or 0. 01 ) five active components could promote the recovery of erythrocytes and hemoglobin; ferulic acid could promote the recovery of leukocyte number and bone marrow hematopoietic tissue area; ferulic acid and astragaloside IV could promote the recovery of platelet. The contents of granulocyte-macrophage colony-stimulating factor ( GM-CSF ), thrombopoietin (TPO), erythropoietin ( EPO) in serum significantly increased in Astragalus-Angelica combination and the active components combination groups ( P < 0. 05 or 0. 01); five components could increase TPO content, ferulic acid and calycosin could increase the contents of GM-CSF and EPO, calycosin glycoside could increase EPO. Astragalus-Angelica combination and the active components combination could significantly increase the colony numbers of granulocyte-monocyte colony fo-ming unit ( CFU-GM ) , megakaryocyte colony forming unit (CFU-MK), erythroid colony forming unit (CFU-E) , burst forming unit-erythroid (BFU-E) ; except for astragaloside IV, the others could increase the number of CFU-GM, five active components could all increase the numbers of CFU-MK, CFU-E, but only ferulic acid could increase the number of BFU-E. Conclusions The main active substances that the combination of Astragalus and Angelica enriches the blood are the above five active components, which exert the synergistic effect through acting on different links of hematopoiesis such as increasing the content of hematopoietic growth factor in blood, promoting the proliferation of hematopoietic progenitor cells,and so on.