Lucy-tag promotes expression of sea lamprey (Petromyzon marinus) olfactory receptor on cell membrane / 中国药理学通报

ABSTRACT

Aim To elucidate the effect of Lucy-tag polypeptide label on the expression of olfactory receptor membrane in sea lamprey ( Petromyzon marinus). Methods Immunocytochemis-try, double luciferase system and calcium flow detection were used to verify the effect of polypeptide label Lucy-tag on olfactory receptors of sea lamprey. Results Polypeptide label Lucy-tag promoted the membrane expression of 13 olfactory receptors out of 20 olfactory receptors, and Lucy-tag did not affect the intrinsic activity of olfactory receptors, nor did it affect the IP3 signaling pathway of G protein coupled receptor (GPCR). Conclusions It provides a favorable technique for finding the ligand of olfactory receptor and lays a good theoretical foundation for the further study of the function of the GPCR and ligand.