Inhibitory functions of SaaB on LPS-induced RAW264. 7 cells via NF-κB-iNOS-NO signaling pathway / 中国药理学通报

ABSTRACT

Aim: To investigate the functional influences of saponins from Anemarrhena asphdeloids Bge (SAaB) on lipopolysaccharide (LPS)-induced RAW264. 7 cells and the regulation of SAaB to NF-κB- iNOS-NO signaling pathway. Methods: The inflammatory cell model in vitro was established using LPS- induced RAW264. 7 cells, and the levels of NO, iN- OS, TNF-α and IL-6 in inflammatory RAW264. 7 cells effected by SAaB were analyzed using Griess and ELISA method respectively. The protein expression levels of NF-κB p65 were measured by Western blot. Results: Compared with control group, LPS (10 mg L-1) -induced RAW264. 7 cells expressed a significant increase in the levels of NO, iNOS, TNF-α, IL-6 and NF-κB p65 protein. SAaB (0.3, 3, 30 mg L-1) obviously reduced the contents of these inflammatory factors (P <0. 01) and the expression of NF-κB p65 protein (P < 0. 01) in LPS-induced RAW264. 7 cells. Conclusion: SAaB can inhibit the functions of LPS-induced RAW264. 7 cells by modulating NF-KB- iNOS-NO signaling pathway.