Resveratrol inhibited hepatic granulama in mice with schistosomiasis by modulating Thl7 and treg responses / 中国药理学通报

ABSTRACT

Aim To observe the anli-granuloma of resveratrol in mice with schistosomiasis japonica and its effect on Thl7 and Treg responses. Methods Forty- five mice infected with S. japonieum cercariae for three weeks were randomly divided into three groups named as infected group ( A ) , resveratrol group ( H ) and praziquantel group ( C). Fifteen normal mice were taken as normal control group (D). At the 9th week post-infection, the granuloma of the liver tissues was observed using HE staining, and the number of neutrophils was calculated. IL-17A, CXCL1, CXCL2, Foxp3 levels in liver tissues were delected by RT-PCR. Thl 7 and Treg responses in spleen were determined by FCM respectively. Splenocytes from healthy mice were stimulated by PBS and SEA respectively. After SEA stimulation, resveratrol and praziquantel were added respectively, and Thl7 and Treg responses were detec ted by FCM. Results Compared to group A, the granuloma was alleviated ( P < 0. 01 ) , the number of neutrophils in single granuloma was reduced ( P < 0.01), Thl7 response significantly decreased (P < 0. 05) and Treg response increased markedly in group B ( P <0.05). The hepatic expression of IL-17A, CXCL1 , CXCL2 levels in group B was lower than that of group A (P< 0.05), and Foxp3 level in group B were higher than in group A(P <0. 01 ). In vitro, resveratrol inhibited the differentiation of Thl7 cells (P < 0. 05 ) and enhanced that of Treg cells after SEA treatment ( P < 0. 05 ). Conclusion Resveratrol inhibits the heptic granulomatous response through down-rcgu- lating Thl7 res()onse and up-regulating Treg response in mice with schistosomiasis.