Differential roles of phosphatidylinositol 3-kinase in mitogen-activated prote in kinase activation by insulin and epidermal growth factor / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12): 57-60, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-857396
ABSTRACT
Aim:
To investigate the roles of phosphatidylinositol 3-kinase (PI3K) in the mitogen-activated protein kinase (MAPK) activation by insulin and epidermal growth factor (EGF).Methods:
Phosphorylated MAPK, Akt (also termed protein kinase B) and total protein level of MAPK were determined by Western blotting using phospho- or non-phospho-state specific antibodies; the roles of PI3K in these signaling transduction pathways were assessed by use of PI3K specific inhibitor wortmannin.Results:
Both insulin and EGF rapidly stimulated MAPK phosphorylation; wortmannin totally blocked the MAPK phosphorylation stimulated by insulin, but not by EGF. By contrast, wortmannin equally inhibited Akt phosphorylation stimulated by both insulin and EGF. Moreover, wortmannin inhibited insulin-stimulated MAPK phosphorylation in a concentration-dependent manner, and the inability of wortmannin to inhibit EGF-stimulated MAPK phosphorylation was not changed with the use of low concentrations of EGF.Conclusion:
PI3K plays different roles in the MAPK activation induced by insulin and EGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmacological Bulletin
Year:
2005
Type:
Article
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