The effect of 15-HETE on the cytosolic Ca2+i in rabbit pulmonary artery smooth muscle cells (PASMC) / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12): 66-69, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-857398
ABSTRACT
Aim:
The purpose of this study was to observe the effect of 15-HETE on rabbit pulmonary artery (PA) vasoconstriction after removing extracellular Ca2+ and on [Ca2+]i in PASMCs and to discuss the mechanisms of cytosolic Ca2+ mobilization induced by 15-HETE.Methods:
We used tention studies of PA rings to observe the effect of L-type Ca2+ channel blocker and non-Ca2+ solution on PA vasoconstriction induced by 15-HETE. Then we used laser-scanning confocal microscope to investigate [Ca2+]i signaling in cultured PASMCs.Results:
L-type Ca2+ channel blocker and non-Ca2+ solution had no effect on 15-HETE induced vasoconstriction in normoxic and hypoxic rabbit PA rings. The increase of [Ca2+]i was shown in 15-HETE group cells and the change in [Ca2+]i induced by 15-HETE was significantly different from that of control.Conclusion:
15-HETE may activate Ca2+ release from intracellular stores and raise [Ca2+]i in PASMCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmacological Bulletin
Year:
2005
Type:
Article
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