Culturing rat Kupffer cell with double collagen culture / 中国药理学通报

ABSTRACT

AIM: To explore a long-term Kupffer cell culture method, which can best maintain the function and activity of cell and decrease the cellular damage. METHODS: Lactic dehydrogenase (LDH) in the supernatants were measured to observe the severity of cellular damage, the function of Kupffer cell was reflected with the nitrogen monoxide(NO) content and the intracellular acid phosphatases(ACP) activity. In the conditions of common culture(CC), single collagen culture(SCC) and double collagen culture(DCC), with or without serum, study the maintenance of cellular function and the severity of cellular damage. RESULTS: Compared with the other two methods, the LDH contents were significantly lower (P < 0.05; P < 0.01), the levels of both NO at different days and the cellular ACP at 15d were markedly increased (P < 0.05; P < (0.01) in DCC group with serum. Even without serum, the cellular damage and function could achieve satisfactory results in DCC group. CONCLUSION: DCC is a useful method to study Kupffer cell in vitro.