Effects of different interval intracerebroventricular administration of brain-derived neurotrophic factor on hypoxic ischemic encephalopathy in neonatal rats / 中国药理学通报

ABSTRACT

AIM: To observe the effects of different interval administration of exogenous brain derived neurotrophic factor (BDNF) on hypoxic-ischemic encephalopathy in neonatal rats. METHODS: BDNF (0.5 μg) was microinjected intracerebroventricularly at 0, 1 and 4 h after the hypoxic-ischemic encephalopathy in 7 d neonatal Sprague-Dawley rats undergone by ligation of left common carotid artery followed by a 2.5 h inhalation of humidified 8% 02 + 92% N2 at 37°C immediately after the injury, respectively. Changes of brain edema, levels of malondialehyde (MDA) and neuronal apoptosis at the left cortex and hippocampus were investigated 24 h after the injury. RESULTS: The most prominent effect of BDNF was seen in 0 h group with a marked decreases in brain edema and levels of MDA and a significantly alleviated neuronal apoptosis while it was lowered obviously when administered at 4 h after the insult. CONCLUSION: BDNF exerts a prominent protective effect on hypoxic-ischemic encephalopathy in neonatal rats when given as early as possible after the injury rather than later administration.