Effect of growth inhibition of diallyl trisulfide on gastric cancer MGC-803 cell line in vitro / 中国药理学通报

ABSTRACT

AIM: To study the effects of growth inhibition of different concentrations of diallyl trisulfide(DATS) on gastric cancer MGC-803 cell line in vitro. METHODS: The influence of different concentrations of DATS were examined by MTT assay, clonal formation rates and cell growth curve. RESULTS: Suppression and decrease of MGC-803 cell proliferation was found after treatment by DATS in vitro. The inhibitory rates on MGC-803 cell growth of different concentration of DATS, 4, 8, 12, 16 and 24 mg·L-1, were 26%, 46%, 65%, 76% and 89% respectively, and its half inhibitory concentration (IC50) was 8.2 mg·L-1. The clonal formation rates and clonal formation relative counts of 8, 12, 16 and 24 mg·L-1 were 32.4% and 58.7%, 24.8% and 42.5%, 19.0% and 33.5%, 8.8% and 15.1% respectively. There was significant correlation between dose and effect in all, and the cell growth culve became lower and flatter when concentration of DATS increase gradually. CONCLUSION: The effect of growth inhibition of DATS for gastric cancer MGC-803 cell in vitro is remarkable.