The effects of a diterpenoid compound 5F isolated from pteris semipinnata L on the activity and expression of mitogen activated protein kinase in K562 cells / 中国药理学通报

ABSTRACT

AIM: To study the effects of a diterpenoid compound 5F (ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic acid) isolated from pteris semipinnata L on the the activity and expression of mitogen activated protein kinase of K562 cells. METHODS: K562 cells were treated with compound 5F for 24 hours, then MAPK activity was determined using MBP as substrate, Western blotting was used to detect the effect of compound 5F on the expression of MAPK. RESULTS: The activity and expression of MAPK increased after the treatment of 5F. CONCLUSION: One mechanism of the anti-tumor effect of compound 5F is through abnormal activation and abnormal expression of MAPK.