Effects of sodium quercetin-7-sulfate on recombinant human protein kinase CK2 holoenzyme / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12): 315-318, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-857495
ABSTRACT
AIM:
To study the effects and kinetics of sodium quercetin-7-sulphate (SQMS) on recombinant human protein kinase CK2 holoenzyme.METHODS:
The recombinant human CK2 holoenzyme activity was assayed by detecting incorporation of 32p of [γ-32P]ATP into the substrate at various conditions.RESULTS:
SQMS was shown to strongly inhibit the holoenzyme activity of recombinant human protein kinase CK2 with an IC50 of 1.37 μmol·L-1, Kinetic studies of SQMS on recombinant human CK2 showed the inhibition was competitive with ATP and noncompetitive with casein.CONCLUSION:
SQMS is an inhibitor of protein kinase CK2, and the inhibitory action of SQMS on CK2 is competitive with ATP and noncompetitive with casein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmacological Bulletin
Year:
2002
Type:
Article
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