Identification and bioinformatic function analysis of p53-related differentially expressed IncRNAs in γ-rays irradiated-human bronchial epithelial HBE cells / 中国药理学与毒理学杂志

ABSTRACT

OBJECTIVE: To identify the differentially expressed long non-coding RNAs (IncRNAs) in γ-rays irradiated p53 knockout HBE (HBEp53-/-) cells, and to provide more baseline information for further investigation of p53 related IncRNAs in irradiation-induced injury, such as epithelial-mesenchymal transition in lung tissue epithelial cells. METHODS: HBEp53-/-cells were established with CRISPR-cas9. Western blotting was used to detect the knockout efficacy of P53 protein, while real time RT-PCR was used to verify the selected expressions of IncRNAs. Bioinformatic analysis including GO and KEGG analysis was used to predict the IncRNA function and signaling pathway. RESULTS: Compared to the irradiated HBE cells, 239 IncRNAs were up-regulated and 289 down-regulated in irradiated HBEp53-/-cells. The top five of the up-regulated or down-regulated differentially expressed IncRNAs were further confirmed by RT-PCR analysis. Through bioinformatic prediction GO analysis, the targeted genes of IncRNAs with changed expressions were related to multiple biological processes and molecular functions, including β3adrenergic receptor binding, protein tyrosine kinase activity, DNA binding, zic ion transmembrane transporter activity, ubiquitin binding and syntaxin binding. KEGG analysis revealed that the functional organismal systems involved included the sensory, nervous, immune, endocrine, environmental adaption, developmental, and circulatory systems. CONCLUSION: p53-related radiation-inducible IncRNAs have been identified in HBEp53-/-cells. These identified IncRNAs may play critical roles in various biological processes and functional signaling pathways in cellular response to radiation injury.