Effect of acetaminophen on thyroxine relative genes of zebrafish embryos / 中国药理学与毒理学杂志

ABSTRACT

OBJECTIVE To investigate the effects of N-acetyl-p-aminophenol (APAP) on zebrafish pigments and thyroxine related genes. METHODS Well-developed zebrafish embryos were exposed to APAP 0, 2, 4 and 8 mmohL-1 from 4 h post fertilization (hpf) and observed at 24, 48, 72 and 96 hpf, respectively, each with 3 replicates. Image J was used to detect the change of pericardial edema of zebrafish. The conentrations of triiodothyronine and tetraiodothyronine were determined by ELISA. mRNA of thyroxine relative genes TPO, Trh, Dio2, Dio3, Thru and Thrf} was detected by fluorescent quantitative PCR. RESULTS At 72 hpf, compared with control group, the hatching rate and pigmentation accumulation decreased in APAP 8 mmol • L-1 group (FcO.OI), but the pericardial area was increased (P<0.01). The conentrations of tetraiodothyronine and triiodothyronine were decreased in APAP 8 mmol • L-1 group (P<0.05). Quantitative PCR results showed that APAP down-regulated mRNA levels of TPO, Trh and Thrfi in APAP 8 mmol-L-1 exposure group (P<0.05). There was no statistically significant difference in the expression of other genes. CONCLUSION The decrease in pigmentation and hatching rate caused by APAP may be related to the decreased expressions of thyroid hormone-related genes.