Quality evaluation of suspended red blood cells stored at high altitude / 中国药理学与毒理学杂志

ABSTRACT

OBJECTIVE To evaluate the impact of the plateau environment on suspended red blood cells (SRBCs). METHODS  Plain SRBCs (from healthy people living in Beijing) were stored in Beijing, Lhasa and Linzhi at 4? for 35 and 42 d, respectively. Plateau SRBCs (from healthy people living in Lhasa) were stored at 4? in Lhasa for 35 and 42 d. Hemolysis rate, hemoglobin content, hematocrit and cell survival rate were examined. The extracellular acidification rate of erythrocytes was detected by the extracellular flow analysis technology before baseline glycolsis, glucose response and glycolysis capacity were calculated.RESULTSCompared with the plain SRBCs preserved in Beijing for 35 and 42 d, there was no statistically significant difference in the hemolysis rate, hemoglobin content, hematocrit, cell survival rate, baseline glycolysis, glucose response or glycolysis capacity of SRBCs stored in Lhasa. In SRBCs stored in Linzhi, except the hemolysis rate and glycolysis ability for 35 d(P<0.05), there was no statistically significant difference in other indicators. Compared with plateau SRBCs preserved in Lhasa, after preservation in Lhasa for 35 and 42 d, plain SRBCs had no statistically significant difference in all the indicators above.;CONCLUSION The plateau environment (Lhasa or Linzhi) does not affect the quality of stored SRBCs.