Effect of Eucommia ulmoides Polysaccharide on db/db Diabetic Mice's Glycolipid Metabolism / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effect and mechanism of Eucommia ulmoides polysaccharide (EUP) on db/db diabetic mice's glycolipid metabolism. METHODS: The db/db type 2 diabetes mice model were randomly divided into model control group, EUP low, middle and high dose group and positive control metformin group,-+/db mice of the same age were set as normal control group. The mice were weighed, and the changes of fasting blood glucose (FBG), blood lipid (TG, TC, LDL-C, HDL-C), interleukin (IL-8, IL-1β, IL-6), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were detected after drug delivery for 9 weeks. HE staining and oil red O staining were used to evaluate the pathological changes and steatosis of liver in mice. Western blot assay were used to detect the protein expressions of NF-κB p65 and HO-1 in liver tissues of mice. RESULTS: EUP could reduce the body weight of db/db mice, reduce the levels of FBG, TG, TC, LDL-C, IL-8, IL-1β, IL-6, MDA, NF-κB p65 proteins and increase the levels of HDL-C and HO-1 proteins. EUP could alleviate pathological changes and steatosis of liver in db/db mice. CONCLUSION: The obesity, hyperglycemia, hyperlipidemia and liver injury of db/db mice with type 2 diabetes are all improved by EUP, and the mechanism is related to the regulation of inflammatory response and oxidative stress.