Effect of Jieyu No.1 Recipe on Neurotransmitter and Notch Signaling Pathway in Hippocampus of Rats After Stroke / 中国药学杂志

ABSTRACT

OBJECTIVE: To analyze the effects of Jieyu No.1(traditional Chinese medicine) on behavior, brain neurotransmitter and hippocampus Notch signaling pathway in post-stroke depression (PSD) rats. METHODS: Sixty Wistar male rats of SPF grade were randomly divided into four groups. The PSD model was prepared by rats in other groups except the normal group. After successful modeling, the rats in the positive control group were intragastrically administered 2.33 mg•kg-1 of fluoxetine hydrochloride solution, Jieyu No.1 prescription group 2 g•kg-1 of Jieyu No.1 prescription liquid, rats in normal group and model group were given equal dose of distilled water, once/d, continuous irrigation Stomach for 8 weeks. The behavioral characterization, neurotransmitter content, Notch protein, Hes protein expression and Notch1 and Hes1 mRNA expression in the brain of each group were observed. The function of Notch signal pathway in hippocampus of each group was detected. RESULTS: Compared with the normal group, the neurological function scores of the model group increased at 1, 4, and 8 weeks after administration; compared with the model group, the positive control group and the Jieyu 1 group were administered 1, 4, and 8 weeks after administration. The neurological function score of rats was decreased, and the difference was statistically significant (P<0.05). Compared with the normal group, the vertical movement, horizontal movement, and cleaning movements of the model group decreased, and the number of feces and the residence time of the central compartment increased. Compared with the model group, the positive control group and the Jieyu 1 group were larger. The vertical movement, horizontal movement, and cleaning movements of the rats increased, the number of stools and the residence time of the central compartment decreased, and the difference was statistically significant (P<0.05). Compared with the normal group, the 5-HIAA in the brain tissue of the model group was the contents of NE, 5-HT and DA were decreased. Compared with the model group, the contents of 5-HIAA, NE, 5-HT and DA in the brain of rats in the positive control group and Jieyu No.1 group were increased. The difference was statistically significant(P<0.05); compared with the normal group, the model group rats increased open time, current amplitude, open probability, and closed time decreased; compared with the model group, positive control group, Jieyu 1 square group rats open time, flow amplitude, open probability increased, reducing the off-time, the difference was statistically significant (P<0.05). CONCLUSION: Jieyu No.1 can increase the neurotransmitter content in the brain tissue of PSD rats and improve the behavioral behavior. The mechanism may be related to the activation of Notch signaling pathway in rat hippocampus, thus accelerating the recovery of nerve function.