Simultaneous Determination of Twelve Constituents in Yigan Jiedu Capsules by HPLC / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 557-561, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-857746
ABSTRACT
OBJECTIVE:
To develop a method for determining 12 constituents in Yigan Jiedu Capsules by HPLC.METHODS:
The quantitative analysis was carried out on a Waters Atlantic T3 C18column maintained at 40 ℃, using mobile phase consisting of acetonitrile(A)and 0.1% phosphoric acid solution(B)by a gradient elution program(0-10 min, 4%→13%A; 10-20 min, 13%→15%A; 20-25 min, 15%→19%A; 25-35 min, 19%A; 35-36 min, 19%→23%A; 36-45 min, 23%→27%A; 45-56 min, 27%→43%A; 56-65 min, 43%→65%A) at a flow rate of 1 mL•min-1. The detection wavelength was set at 270 nm in the first 22 min, and then changed to 290 nm between 22 and 44 min, and last changed to 270 nm.RESULTS:
The linear ranges of gallic acid, vanillic acid, picroside Ⅱ, astilbin, engeletin, picroside, baicalin, berberine hydrochloride, wogonoside, emodin, chrysophanol and wogonin fell with in the range of 0.012 19-0.243 8 μg, 0.036 70-0.734 0 μg, 0.033 77-0.675 4 μg, 0.057 38-1.147 6 μg, 0.006 580-0.131 6 μg, 0.015 38-0.307 6 μg, 0.067 11-1.342 2 μg, 0.028 04-0.560 8 μg, 0.041 48-0.829 6 μg, 0.006 310-0.126 2 μg, 0.013 64-0.272 8 μg and 0.014 22-0.284 4 μg. The recoveries were 98.7%, 98.9%, 99.4%, 99.2%, 98.9%, 99.1%, 98.9%, 99.6%, 99.3%, 98.9%, 98.6% and 99.0%, respectively. The relative standard deviations were 1.02%, 0.46%, 0.64%, 0.86%, 1.1%, 1.3%, 0.60%, 0.92%, 0.61%, 1.5%, 0.93%and 0.98%, respectively(n=6).CONCLUSION:
This method is simple, accurate, reproducible and convenient for the quality control over Yigan Jiedu Capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2020
Type:
Article
Similar
MEDLINE
...
LILACS
LIS