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Chemometric Analysis of HPLC Fingerprint of Triterpenoids in Different Ganoderma Preparations Based on Reference Drug / 中国药学杂志
Chinese Pharmaceutical Journal ; (24): 298-304, 2020.
Article in Chinese | WPRIM | ID: wpr-857775
Responsible library: WPRO
ABSTRACT

OBJECTIVE:

To establish a method combining triterpenoids fingerprint with chemometric analysis based on reference drug, and determine 89 batches of samples to evaluate the quality of Ganoderma preparations.

METHODS:

The samples were extracted by ultrasonic with methanol, chromatography was performed on Agilent TC C18(2) column (4.6 mm×250 mm, 5 μm) with gradient elution with acetonitrile and 0.02% phosphoric acid aqueous solution. The detection wavelength was set at 254 nm. Principal component analysis and HCA heatmap were used for data analysis.

RESULTS:

The chromatographic fingerprint has 56 characteristic peaks. By comparing the samples with the reference drug, it was found that Ganoderma preparations were produced using Ganoderma lucidum by almost all manufacturers. Principal component analysis, HCA heatmap analysis and similarity analysis divided the samples into three categories. The first type had characteristic peaks and peak areas which were consistent with the reference drug, and the quality was better. The second type had characteristic peaks which consistent with the reference drug, but the peak areas were smaller and the quality was medium. And the third type was different from the reference drug, which was the problem sample. The strong characteristic peaks for classification of different samples were peak 26, peak 31, peak 24, peak 32 and peak 18.

CONCLUSION:

The method is comprehensive, accurate and specific, and it can be used for comprehensive quality evaluation of triterpenoids in Ganoderma preparations.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Pharmaceutical Journal Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Pharmaceutical Journal Year: 2020 Type: Article