Effect of Trans-oleic Acid on Proliferation Inhibition and Apoptosis Induction in H9c2 Cardiomyocyte / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 199-205, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-857785
ABSTRACT
OBJECTIVE:
To investigate the effects and possible mechanism of trans-oleic acid (9t-C181) on proliferation inhibition and induction apoptosis in H9c2 cardiomyocyte.METHODS:
H9c2 rat cardiomyocytes were cultured in vitro. High, medium and low (600, 300, 150 μmol•L-1) dose of 9t-C181 groups and the negative control (NC) group were administered to H9c2 cardiomyocytes. The effect of 9t-C181 on cell proliferation was tested using cell counting kit-8 (CCK-8) assay. Morphological changes of cells were observed by AO-EB staining. Intracellular reactive oxygen species (ROS) and apoptosis were detected by flow cytometry. The expression of Bcl-2 and Bax genes was detected by quantitative real time- polymerase chain reaction (QRT-PCR). The expression of Bcl-2 and Bax protein was determined by flow cytometry after immunofluorescence staining.RESULTS:
The typical morphological characteristics of apoptosis were observed by fluorescence microscope. The result of CCK-8 assay indicated that 9t-C181 have an certainly inhibitory effect on the proliferation of H9c2 cells. ROS level and apoptosis rate were significantly increased. Bcl-2 gene and protein expression were down-regulated, and Bax gene and protein expression were up-regulated, compared with NC group(P<0.05, P<0.01).CONCLUSION:
9t-C181 can inhibit the proliferation and induce the apoptosis of H9c2 cardiomyocyte, and its mechanism may be related to promoting the mitochondrial apoptotic pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2020
Type:
Article
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