Inhibition Effect of Selenium-Enriched Bifidobacterium longum on Alcohol-induced Liver Damage in Mice / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the effect of selenium-enriched Bifidobacterium longum on alcoholic liver damage and its mechanism. METHODS: Alcoholic liver damage of female C57/BL6 mice were induced with 20% alcohol for 42 d, at the same time 50 or 100 mg•kg-1 selenium-enriched Bifidobacterium longum were administrated respectively. Mice livers were collected for calculating liver index, HE staining and measuring liver tissue malondialdehyde (MDA) and superoxide dismutase (SOD) level. Serum were collected for measurement of alanine transarninase (ALT), aspartate aminotransferase (AST),γ-glutamyltransferase (GGT) and tumor necrosis factor α (TNF-α) levels. The mRNA levels of liver SREBP-1c, AMPK and PPAR-α were measured with Real-time PCR. RESULTS: Selenium-enriched Bifidobacterium longum inhibited the increase in liver index and inflammation index induced with alcohol. Selenium-enriched Bifidobacterium longum also inhibited alcohol induced ALT, AST, GGT and TNF-α levels increase; decreased liver MDA levels and increased liver SOD levels. Selenium-enriched Bifidobacterium longum down-regulated SREBP-1c mRNA and up-regulated AMPK and PPAR-α mRNA levels of mice liver. CONCLUSION: Selenium-enriched Bifidobacterium longum protects mice from alcohol induced liver damage in a dose dependent manner. Its protective mechanism might be anti-oxidation, inflammation inhibition and improvement of lipid metabolism.