Multi-component Quantification Analysis and Overall Quality Evaluation of Dieda Pills / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an HPLC method for simultaneous determination of nine components, i.e., paeoniflorin, naringin, hesperidin, neohesperidin, paeonol, asperosaponin , glycyrrhizic acid, curcumin and acetyl-11-keto-beta-boswellic acid, and evaluate the overall quality of Dieda pills. METHODS: The analysis was performed on an Agilent 1260 Infinity LC System with a diode array detector. The chromatographic separation was performed on Agilent Poroshell 120 EC-C18 (4.6 mm×100 mm, 2.7 μm) column. The mobile phase was a mixture of acetonitrile (mobile phase A) and water containing 0.1% phosphoric acid aqueous solution (mobile phase B). The gradient elution program was as follows 5%-18%A for 0-7 min, keeping 18%A for 7-15 min, 18%-35%A for 15-27 min, 35%-60%A for 27-32 min, 60%-95%A for 32-42 min, keeping 95%A for 42-45 min.The flow rate was set at 1.0 mL•min-1, the column temperature was maintained at 30 ℃ and the injection volume was 5 μL. The detection wavelength was set at 230 nm for paeoniflorin, 283 nm for naringin, hesperidin and neohesperidin, 274 nm for paeonol, 212 nm for asperosaponin , 251 nm for glycyrrhizic acid, 440 nm for curcumin and 251 nm for acetyl-11-keto-beta-boswellic acid, respectively. RESULTS: All the nine components achieved good separation.The linear ranges fell with in the range of 0.1-1.0 μg for paeoniflorin, naringin, neohesperidin andacetyl-11-keto-beta-boswellic acid, 0.2-2.0 μg for hesperidin and asperosaponin , 0.04-0.4 μg for paeonol, 0.02-0.2 μg for glycyrrhizic acid and 0.01-0.1 μg for curcumin,respectively(r2≥0.999 8). The average recoveries (n=6) were 96.95%-100.4% and the RSDs were 0.21%-0.81%. CONCLUSION: The developed method is simple, accurate, reliable, and can be used for the overall quality control and quality evaluation of Dieda pills.