Effects of baijiezi powder and its disassembled formula on the ig-e, il-4, ifn-γ and tnf-α in allergic asthmatic rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effects of baijiezi powder and its disassembled formula on the immunoglobulin (Ig)-E, interleukin (IL)-4, interferon (IFN)-γ and tumor necrosis factor (TNF)-α in local skin of applied drugs and lung tissues of rats with allergic asthma. METHODS： Eighty rats were randomly divided into normal control group, model control group, positive control group, baijiezi powder acupoint group, baijiezi powder non-acupoint group, Semen Sinapis Albae acupoint group, Corydalis Rhizoma acupoint group, Radix Kansui acupoint group, asarum acupoint group and Ginger acupoint group, 8 in each group. In addition to the blank control group, the other nine groups were sensitized by ovalbumin (OVA) to establish a model of allergic asthma. The morphological changes of lung tissue were observed by HE staining. The contents of the Ig-E, IL-4, IFN-γ and TNF-α in local skin of application and serum were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS： The infiltration degree of inflammatory cells was obviously reduced and the alveolar structure was basically normal in baijiezi powder acupoint group, which was similar to the normal control group. The levels of the Ig-E, IL-4, IFN-γ and TNF-α in serum can be recalled in each treatment acupoint group. Especially, baijiezi powder acupoint group, Semen Sinapis Albae acupoint group and asarum acupoint group were the best (P < 0.01). The level of Ig-E in the local skin for the drug application of baijiezi powder acupoint group, Semen Sinapis Albae acupoint group and asarum acupoint group was lower than the normal value. CONCLUSION： Baijiezi powder can effectively treat allergic asthma, can signifcantly enhance the IFN-γ and weaken the expression of Ig-E, IL-4 and TNF-α in the local skin of the application and serum. Meanwhile, the correction intensity of each index value in local skin of applied drugs of baijiezi powder was higher than that of serum. Among all the disassembled herbs, Semen Sinapis Albae and asarum have a strong effect on Ig-E, TNF-α and IFN-γ, while Corydalis Rhizoma mainly has an effect on IL-4.