Identification of Uygur Herb Capparis spinose Based on Sequences of the Plastid psbA-trnH Intergenic Regions / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 965-970, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-857985
ABSTRACT
OBJECTIVE:
To provide a reference for molecular biology identification of C. spinosa by comparing and analyzing the sequence of psbA-trnH gene intergenic regions between Capparis spinosa L. and others 8 medicinal plants.METHODS:
Total DNA were extracted, psbA-trnH intergenic regions sequences were isolated using PCR amplification, and sequence analysis, evaluation of intraspecific and interspecific genetic distance used the Kimura 2 parameter(K2P)model as well as construction of phylogenetic tree based on UPGMA were conducted by the MEGA7.RESULTS:
Compared with C. spinosa, the interspecific genetic distance is 0.045-0.474. The average is 0.238. The interspecific minimum was larger than the intraspecific maximum. The results of UPGMA tree indicated every species was sorted out and C. spinosa was distinguished from the others effectively.CONCLUSION:
The sequences of chloroplast psbA-trnH gene intergenic regions is valuable for the identification and study of C. spinosa.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2019
Type:
Article
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