Identification of Gentiana rhodantha and Its Adulterants by DNA Barcoding Coupled with High Resolution Melting Analysis / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 687-692, 2019.
Article
in Zh
| WPRIM
| ID: wpr-858013
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: To apply DNA barcoding coupled with high resolution melting analysis to distinguish Gentiana rhodantha from its adulterants. METHODS: The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. RESULTS: The ITS2 molecular regions coupled with HRM analysis can effectively differentiate five herbal species, including two Gentiana rhodantha and their four common adulterants. CONCLUSION: DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which may contribute to the quality control of traditional Chinese medicine in the natural health product industry.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Pharmaceutical Journal
Year:
2019
Type:
Article