Rapid determination of teicoplanin concentration in human plasma by uplc and its application / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 484-488, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-858045
ABSTRACT
OBJECTIVE:
To establish a rapid ultra-high performance liquid chromatography method for determining teicoplanin concentration in human plasma.METHODS:
The samples were chromatographed on Waters Acquity UPLC BEH-C18 column using gradient elution method(mobile phase A20 mmol•L-1 ammonium acetate;mobile phase Bacetonitrile). The absorption wavelength was set at 215 nm. The column temperature were maintained at 35 ℃. The teicoplanin concentration was calculated by internal standard method and external standard method, respectively. The results of the two methods were compared by paired t-test using SPSS statisticals software.RESULTS:
The liner range of the calibration curve for teicoplanin was 3.15-100 μg•mL-1(r=0.999 9). The concentrations calculated by the two methods had good correlation without significant difference.CONCLUSION:
This method is proved to be rapid, sensitive and suitable for the therapeutic drug monitoring and pharmacokinetic investigation of teicoplanin in human plasma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2019
Type:
Article
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